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Next, the samples were centrifuged at 3,000 rpm for 10 min at 4 C in an AF-2724 rotor (Kubota, Tokyo, Japan)

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Alcohol stays out of the picture

This approach led to a surge in youth e-cigarette use that FDA is now belatedly scrambling to address. Rather than looking to FDA as the standard-bearer for public health concerns about tobacco, Berman posits that state and local policymakers will be the most effective regulators