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Blog In Summary: Cigarette smoke contains over 7,000 chemical compounds, including about 250 harmful and 69 carcinogenic chemicals, such as carbon monoxide and arsenic, which can cause cancer and other serious health issues

However, while valuable, this model had key limitations, including a lack of reproducible smoke dose delivery, the inability to assess cigarette smoke-induced toxicity in the distal lung, and the absence of essential readouts like trans-barrier electrical resistance (TER), restricting its applicability for studying alveolar-level injuries

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BMC Med (2015) 13(1):24