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Other government initiatives against tobacco have included a 25% excise in 2010, restrictions on Internet advertising, and more than $85 million in anti-smoking social marketing campaigns

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More than 20,000 square miles of land is taken up around the world to accommodate tobacco farms, which use more than 22 billion tonnes of water, meaning a person smoking a pack of 20 cigarettes a day for 50 years is responsible for 1.4 million litres of water depletion over their lifetime

The supernatants of BALF containing total leucocytes were measured in Neubauer counting chamber, and the remaining sample was stored at 80C