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Starting doses may be reduced in these patients

Quantitative real-time PCR analysis Total RNA was isolated by TRIzol Reagent (Life Technologies, Gaithersburg, MD) following the manufacturers instructions [42]

Proteins were separated by SDS-PAGE at 100 V for 90 min in Tris/glycine/SDS running buffer, then transferred onto polyvinylidene difluoride (PVDF) membranes (#1,620,177, BIO-RAD, Warsaw, Poland) using a wet transfer system at 100 V, 250 mA for 1 h at 4 C in Tris/glycine buffer containing methanol, as previously described by Alicka M

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