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The presynaptic ribbons (red channel) were identified through segmentation, then quantified and tracked in the z-dimension to avoid superimposition ambiguity or overestimation in each stack

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Additionally, the DII itself, while validated, does not capture all aspects of diet quality, such as food processing (NOVA classification), glycemic load, or meal timing, and its calculation may not be easily transferable to all cultural contexts without adaptation of food composition databases

These values are the estimated amount of niacin that most people (97.5%) need to get from their diets every day