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The parameters of GO analysis were set as follows: min overlap = 3, p-value cutoff = 0.05, min enrichment = 1.5

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A needle that has been through skin once develops microscopic burrs on the tip that increase pain, tissue damage, and infection risk with each subsequent use

Amino acids can be arranged in any order you like, so a chain of 15 amino acids has 20^15 possible sequences, or about 3.3 x 10^19, which is 33 quintillion possibilities