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The famous antibody 30K, used since 1975 in Ungers and many other laboratories [16, 17], is directed against the C-terminus of glucagon and, therefore, does not show major cross-reactivity with the gut-derived products of the glucagon gene (only the glucagon 1-61 peptide from the pancreas can interfere with C-terminal-targeting assays, but this circulates in low concentrations and is secreted in parallel with pancreatic glucagon [18])