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The concentration of IL-1 and TNF- from the serum was determined by comparing them to the standard curves provided in the ELISA kit and expressed as pg./mg protein ( 2.8.3 Lipid peroxidation Tissue malondialdehyde was measured as an index of lipid peroxidation using the assay of thiobarbituric acid reacting substances (TBARs) as described by Nagababu ( 2.8.4 Reduced glutathione Reduced glutathione (GSH) was measured in the aorta, heart, and kidney supernatants using Ellmans reagent in a method described by Jollow ( 2.8.5 Catalase enzyme assay The colorimetric assay based on the yellow complex with molybdate and HO 2 was used to measure the amount of catalase present in the tissues of the kidney, heart, and aorta, which was described by Goth and others ( 2 O 2 in sodium potassium phosphate buffer (60 mM, pH 7.4)

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Chest tightness or shortness of breath at faster infusion rates

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