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1208 Additionally, the development of the CRISPR-human Organoids-Single-cell RNA Sequencing (CHOOSE) system offers a comprehensive screening approach for functional loss in organoids, thus providing a detectable pathway for precise metabolic regulation of diseases

Notably, human genome-wide association study (GWAS) data have shown that single-nucleotide polymorphisms in the vicinity of the TGFBI gene are associated with type 1 diabetes risk, and TGFBI has been shown to promote islet survival, function and regeneration in mice [43]

This finding aligns with prior research showing that fasting interventions can upregulate antioxidant enzymes and reduce markers of oxidative stress 21,36

Its regulatory relevance includes controlled applications in research settings