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Samples were processed for protein isolation using RIPA buffer (Boston Bio-product, Ashland, MA) with protease inhibitors cocktail (Cell signaling technology, Danvers, MA), quantified by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) and 40 g of proteins were boiled with 4 denaturing Laemmli Sample Buffer (Bio-Rad Laboratories, Hercules, CA) and loaded on 7.5% SDS-PAGE gel

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According to van Egmond et al