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HEK 293T cells (Cell Bank at the Chinese Academy of Sciences) were cultured in Dulbeccos modified Eagles medium (DMEM, Life Technologies) supplemented with 10% fetal bovine serum (FBS, Gibco) and maintained in a humidified chamber with 5% CO 2 at 37 C

The final nano construct (PLGA/AuNR/DTX@MnO 2 ) was assessed for theranostic applications in a breast cancer cell line, xenograft mice model, in vitro toxicity evaluation, and dual-modal imaging

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