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Measurement of Lipid Peroxidation Lipid peroxidation level in the brain cell homogenate was quantified using the thiobarbituric acid reactive substances (TBARS) as previously described (Buege and Aust, 1978) and using the BMG Fluostar Optima multimode plate reader (NY, USA)

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The main beneficial effects related to coffee consumption are antioxidant, antifibrotic, insulin-sensitizing, and anticarcinogenic

Next increasing volumes (2, 5, 10, 15, 20, 25 L) of the solution of 0.250 mol/L TCEP were added to the samples to obtain the required molar excess of TCEP in relation to N-Hcy-Lys (50:1, 125:1, 250:1, 375:1, 500:1, 625:1)