Cellular energy
Because Crohns causes inflammation of the intestinal tract, it not only interferes with B12 absorption but can cause more severe symptoms like arthritis, skin problems, fevers, rectal bleeding, and abdominal pain
Reported applications include NNMT enzyme inhibition assays measuring 1-methylnicotinamide formation or S-adenosylhomocysteine release, intracellular target-engagement assays in differentiated 3T3-L1 adipocytes where 5-Amino-1MQ reduced intracellular 1-methylnicotinamide with an EC near 2.3 M (Neelakantan et al., Biochem Pharmacol, 2018), membrane permeability and Caco-2 transport characterisation, methyltransferase selectivity panels covering COMT, DNMT1 and PRMT3, and rodent studies of NNMT inhibition in adipose tissue and liver following the antisense knockdown work of Kraus and colleagues (Nature, 2014)
If I have any of this wrong, feel free to correct me (addressing more experienced members of this forum) The problem with a retest at this point is that after 6 injections, the B12 level will likely be elevated and it will be very difficult to know what the original value would have been
