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After endogenous peroxidase activity was blocked by incubation with 3% hydrogen peroxidase in methanol for 30 min, antigen retrieval was achieved by microwaving the sections in citrate buffer for 20 min, and the samples were then incubated overnight at 4C with a mouse monoclonal anti-human GLP-1R antibody (clone 419208

Use bacteriostatic water (not sterile water) to extend solution stability after reconstitution