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Collect the supernatant, 1:1 with nickel column adsorption buffer (10mM imidazole, 50mM NaCl, 50mM Tris-HCl pH8.0) (mixed), nickel column adsorption (prepacked column, commercially available), twice the column volume adsorption Buffer washing, 20mM imidazole buffer (20mM imidazole, 50mM NaCl, 50mM Tris-HCl pH8.0) was washed again to remove impurities

CircPVT1 up-regulation attenuates steroid-induced osteonecrosis of the femoral head through regulating miR-21-5p-mediated Smad7/TGF signalling pathway

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