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Mol Cell Proteom 20:1000022 Neuzil J, Dyason JC, Freeman R, Dong L-F, Prochazka L, Wang X-F, Scheffler I, Ralph SJ (2007) Mitocans as anti-cancer agents targeting mitochondria: lessons from studies with vitamin E analogues, inhibitors of complex II

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2.7 Aortic ring assay The mouse aortic ring assay was conducted according to a previously described method ( 2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes