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The released cells were washed and resuspended in DMEM containing 10% fetal bovine serum (FBS, Sanko Junyaku Co., Tokyo, Japan, lot number: 27110307) and plated at a density of 110 6 cells per 100 mm dish for primary monolayer cultures, or 110 6 cells per 35 mm dish for calcium influx assay and immunocytochemical assay of nAChR

Understanding the differences between cigars and cigarettes can help individuals make informed choices about their tobacco consumption

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Human trafficking is the illegal exploitation of a person