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In order to elucidate the target for free inositols, we propose an in vitro study of cultured hepatocytes or myocytes, as highly-responsive cells to insulin stimulus, and the blockade, one by one, of elements composing the cascade of the insulin signaling pathway (IR, IRS1, PI3K, PDK, Akt, mTOR, and AMPK) in a top-down manner

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Surgery 114:147153 Rhoads JM, Argenzio RA, Chen WU, Rippe RA, Westwick JK, Cox AD, Berschneider HM, Brenner DA (1997) L-glutamine stimulates intestinal cell proliferation and activates mitogen-activated protein kinases