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PR619 (Sigma, 662141) and P5091 (MCE, HY-15667) 44,45 were used to inhibit cysteine DUBs and USP7 specifically, while the proteasome was inhibited using Bortezomib (Sigma, 5043140001)

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The main difference from proteins lies in their length and structure: peptides generally have molecular weights below 5000 Da and often lack stable three-dimensional structures, whilst proteins contain more than 100 amino acids, exceed 5000 Da in molecular weight, and fold into complex three-dimensional conformations with multiple functional domains

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