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Prior to starting the study, the investigators used PASS software (version 11) to calculate minimum sample size 11

GSH prevents LOsG-induced pancreatic fibrosis via the TGF/SMAD pathway in vivo Considering the crucial role of TGF-1 in organ fibrosis, we examined whether LOsG contributed to TGF-1 expression by detecting TGF-1 protein levels in pancreatic tissues using western blotting

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After incubation, each well was washed with dH 2 O, after which 200 l of RPMI 1640 nystatin (1 g/ml) OligoG (4%) was added to each well and incubated for 3 h at 37C (n=3)