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Accessory proteins such as zonula occludens (ZO)-1, ZO-2, AF-6, 7H6, and cingulin function as essential molecular bridges connecting transmembrane tight junction proteins to the intracellular actin cytoskeleton, thereby playing a critical role in preserving the structural and functional integrity of tight junctions (148)

Meanwhile, to substantiate the protective role of serotonin in experimental APAP-induced liver injury, we reloaded Tph1-/- mice with serotonin by injecting its precursor 5-HTP 3 days beforehand