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The peptide's presence in nearly every cell type hints at its fundamental biological importance

Expression of genes encoding alanine transporters in the cARLA-treated human co-culture model of BBB are shown from our previously published dataset publicly available at the Gene Expression Omnibus (GEO) under accession number GSE224846 [40]

NAC and glutathione synthesis Allen & Bradley (2011), Journal of Alternative and Complementary Medicine , found that NAC supplementation increased glutathione-related antioxidant markers, supporting its role as a precursor in the body's own glutathione production

Quantitative real-time PCR RNA was extracted from whole brain using TRI Reagent and PureLink mini-RNA kit (Thermo Fisher Scientific, Cat # 12183020) according to the manufacturers protocol (Thermo Fisher Scientific)