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Option 2: Upper/Lower Split (Intermediate) Monday/Thursday: Upper body (chest, back, shoulders, arms) Tuesday/Friday: Lower body (legs, glutes, core) Each workout 45-55 minutes

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The tissue was then incubated in Hanks buffered salt solution (HBSS) containing 15 mM HEPES, 50 U/mL collagenase type II (Thermo Fisher 17101015), and 100 U/mL hyaluronidase (Sigma Aldrich H3506) at 37 C for 30 min