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Monitoring the effluent at 220 nm, chromatography (flow rate: 18.0 mL/min) was performed starting with a MeOH/H 2 O mixture (85/15, v/v) for 1 min, increasing the MeOH content to 100% within 10 min, holding for 10 min, followed by a decrease of the MeOH content to 85% within 1 min and re-equilibration for 2 min at 85% MeOH

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Four CCK proteoforms were significantly enriched in the frail brain and negatively associated with cognitive Z-score (Fig