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The TBI was induced via an impactor fitted with a flat metal tip, applying parameters of 3 m/s speed, 0.2 s of contact time, and a penetration depth of 1.5 mm

Once cells were proliferating, they were moved up to higher cell volumes and adapted to DMEM cell culture media with 20% dFBS, 50 U ml 1 penicillin and streptomycin and 1 mM sodium pyruvate for at least 1 week before experimental setup

GHK-Cu (Copper Tripeptide-1) Dosage The typical dosage for GHK-Cu varies depending on the mode of administration

Beer C, Foldbjerg R, Hayashi Y, Sutherland DS, Autrup H (2012) Toxicity of silver nanoparticles nanoparticle or silver ion