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HGF Hepatocyte Growth Factor aCSF was removed and the slices were lysed using RIPA buffer (Upstate/Mi lipore, Billerica, MA) and inhibitor Cocktails I and II (Sigma, St.Louis, MO), sonicated on ice and clarified by centrifiigation for 30 minutes, 13,000 rpm at 4C

High performance liquid chromatography and mass spectrometry Synthetic GS-MQ or APR-246 treated de-proteinized JH-EsoAd1 cell lysates were resolved on an ODS-Hypersil C18 HPLC column (2.1 100 mm, 5 m, Hewlett-Packard) in 10 mM NH 4 HCO 3 pH 8, using a 080% acetonitrile gradient over 10 min

In addition, gene expression of PDEs has significant tissue specificity (e.g., PDE3A is mainly expressed in the heart and vascular smooth muscle)

this includes coronary heart disease, cerebrovascular disease, peripheral arterial disease [51, 52]