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High-throughput automated preparation and simulation of membrane proteins with HTMD

a , Scheme of the in vitro sgRNA competition assay performed in HEK-293T SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with a pool of 5 control sgRNAs (sgControl, gray) and sgRNAs targeting TXNRD2 (pink) and SLC25A40 (green) (left)

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These included mechanical tissue damage simulations, chemically induced gastric lesions, vascular interruption models, and inflammation-driven structural breakdown in animal subjects