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We also measured the increase in ROS generation in the nerve ring of the adult worm, caused by the treatment with 1 mM quercetin for 3 h, by repeated-measures ANOVA at different time points (after 1, 3, 5, and 7 min of DCFDA-treatment) and found a significant increase in pixel intensity (indicating increased staining with the ROS-detecting dye, DCFDA) with time (Fig