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Ansar H, Mazloom Z, Kazemi F, Hejazi N
Cellular uptake study FaDu human head and neck cancer cells, LOVO colon carcinoma cells, and T24 bladder carcinoma cells (American Type Culture Collection, Manassas, VA, USA) were maintained in Eagles Minimum Essential Medium (Sigma-Aldrich, St Louis, MO, USA), Nutrient Mixture F-12 Ham Medium (Sigma-Aldrich) and McCoys 5A Medium (Sigma-Aldrich), respectively, supplemented with 10% fetal bovine serum and penicillin (100 U/mL)-streptomycin (100 g/mL) at 37 C in a humidified atmosphere of 95% air and 5% CO 2 (normoxic condition) in 6-well flat bottom plates (Corning Costar, Corning, NY, USA)
Everything below describes a documented research protocol , not a prescription or a personal recommendation