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which is similar to the lactose metabolite allolactose
It may be used to purify DNA binding proteins
coli during plasmid amplification
the bacterial cell wall becomes weak and cytolysis occurs
IPTG Size:200 g which is similar to theIPTG is an analog of galactose that is non metabolizable and inactivates the lac repressor to induce synthesis of galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria. IPTG is commonly used in cloning procedures that require induction of galactosidase and is most often used with
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