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For the validation cohort (2021), a similar protocol than above was used in 96 well plates with the following modifications: Digestion was made with 0.25 g of trypsin only, acidification was performed with formic acid 1% (FA)

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Given this information, emerging therapies which target GSH homeostasis such as N-acetylcysteine (NAC), GSH esters, or activation of antioxidant response elements may provide new avenues to interrupt oxidant-driven inflammation in these diseases