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Likewise, our in vitro study showed that GLP-1R protein level in HUVECs was significantly decreased after exposure to PA for 24 h

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Two hundred and fifty ng of purified RNA were reverse transcribed into cDNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems) and relative mRNA expression determined using FAST SYBR green chemistry qPCR and Applied Biosystems 7500 Real-time PCR system