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Morning administration in a fasted state is the most commonly cited timing because endogenous fat mobilisation is naturally elevated after an overnight fast, theoretically amplifying the lipolytic signal from the peptide

Moreover, KO mice for liver IGF-1 gene developed muscle insulin resistance (it is important to mention that skeletal muscle myocytes express high number of IGF-1R), showing an increase in insulin concentrations and a substantial decrease in the insulin-induced autophosphorylation of the insulin receptor and IRS in skeletal muscle (being normal in liver and white adipose tissue)