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The key variables are form, dose, and timing

For example, water-soluble vitamins often act in the cytosol of cells (the fluid inside of cells) or in extracellular fluids such as blood, while fat-soluble vitamins play roles such as protecting cell membranes from free radical damage or acting within the cells nucleus to influence gene expression

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Under the prevailing nutritional conditions of dairy farms in the Colombian high tropics, it is likely that the negative energy balance (NEB) during the transition period forces cows to increase the use of amino acids for energy, diminishing the lysine and methionine pools, which are substrates for carnitine synthesis